Katri Eskelin


Description of research and teaching

Novel purification and analysis methods for macromolecular complexes.

Asymmetric flow field-flow fractionation (AF4) is a subtechnique of field-flow fractionation. It separates molecules according to differences in their diffusion coefficients, which in turn reflects their size and shape. AF4 is applicable to a broad range of molecular masses and sizes and molecules with sizes that range from 2 nm to 50 μm can be analyzed.

My research focuses on the development of optimized methods to purify large macromolecular complexes such as ribosomes (a complex of 80 ribosomal proteins and three ribosomal RNAs), viral assemblies as well as intact viruses of various sizes and morphologies. Furthermore, my research aims to use AF4 for detailed analysis of large macromolecular complexes. This can be achieved by analyzing complexes that have been treated with various chemicals, enzymes, temperatures etc.

I am combining the AF4 purification to other methods that include monolithic anion chromatography and centrifugation methods to enable large-scale purification of macromolecular complexes to detailed biochemical and structural analysis.

My previous research included studies on two different plant viruses (Cocksfoot mottle virus and Potato virus A) and applied projects on heterologous protein production in plants.  


Latest publications

  1. Ribosome profiles and riboproteomes of healthy and Potato virus A- and Agrobacterium-infected Nicotiana benthamiana plants

    Eskelin, K., Varjosalo, M., Ravantti, J. & Makinen, K., 2019, In : Molecular Plant Pathology. 20, 3, p. 392-409 18 p.

    Research output: Contribution to journalArticleScientificpeer-review

  2. Controlled Disassembly and Purification of Functional Viral Subassemblies Using Asymmetrical Flow Field-Flow Fractionation (AF4)

    Eskelin, K. J. & Poranen, M. M., Nov 2018, In : Viruses. 10, 11, 13 p., 579.

    Research output: Contribution to journalArticleScientificpeer-review

  3. Asymmetrical flow field-flow fractionation in purification of an enveloped bacteriophage φ 6

    Lampi, M., Oksanen, H. M., Meier, F., Moldenhauer, E., Poranen, M. M., Bamford, D. H. & Eskelin, K., 15 Sep 2018, In : Journal of Chromatography. B. 1095, p. 251-257 7 p.

    Research output: Contribution to journalArticleScientificpeer-review

View all (21) »

Latest activities

  1. Asymmetrical flow field flow fractionation services provided by Instruct-FI Biocomplex unit

    Katri Eskelin (Speaker)
    15 Nov 2018

    Activity: Talk or presentation typesInvited talk

  2. Joint Annual Meeting of Finnish Synchrotron Radiation User Organisation (FSRUO) and Finnish Structural Biology Network (FinnBox)

    Katri Eskelin (Attendee)
    15 Nov 201816 Nov 2018

    Activity: Participating in or organising an event typesOrganisation and participation in conferences, workshops, courses, seminars

  3. Analytical Chemistry (Journal)

    Katri Eskelin (Reviewer)
    1 Oct 2018

    Activity: Publication peer-review and editorial work typesPeer review of manuscripts

View all (20) »

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