Research outputs

  1. Data supporting the regulation of FOXC2 in podocyte dysfunction

    Abstract This data article shows the expression levels of specific podocyte injury markers and podocyte slit diaphragm protein nephrin in obese and lean Zucker rat glomeruli. It also contains information on the effect of the overexpression of transcription factor FOXC2 on the ratio of F- and G-actin and the expression level of ZO-1 in differentiated human podocytes. The article also shows data on the effect of treatments of differentiated podocytes with various factors associated with obesity and diabetes on the expression level of FOXC2. The detailed interpretation of these data and other aspects of podocyte injury mediated by upregulation of FOXC2 can be found in “Overexpression of transcription factor FOXC2 in cultured human podocytes upregulates injury markers and increases motility [1].

    General information

    StatePublished
    Ministry of Education publication typeA1 Journal article-refereed
    OrganisationsHaartman Institute, Medicum
    AuthorsDatta, N., Lindfors, S., Miura, N., Saleem, M. A., Lehtonen, S.
    Pages514 - 520
    Publication date2016
    Peer-reviewedYes

    Publication information

    JournalData in Brief
    Volume6
    ISSN (Print)2352-3409
    Original languageEnglish
    Fields of Science3111 Biomedicine
    Electronic versions
    DOIs
    SourceBibtex
    Source-IDurn:e0d736266a359a94e205e93ce98b833a

    Research output: Scientific - peer-reviewArticle

  2. Combinatorial treatment with oncolytic adenovirus and helper-dependent adenovirus augments adenoviral cancer gene therapy

    Oncolytic adenoviruses (Onc.Ads) produce significant antitumor effects but as single agents they rarely eliminate tumors. Investigators have therefore incorporated sequences into these vectors that encode immunomodulatory molecules to enhance antitumor immunity. Successful implementation of this strategy requires multiple tumor immune inhibitory mechanisms to be overcome, and insertion of the corresponding multiple functional genes reduces the titer and replication of Onc.Ads, compromising their direct ant-tumor effects. By contrast, helper-dependent (HD) Ads are devoid of viral coding sequences, allowing inclusion of multiple transgenes. HDAds, however, lack replicative capacity. Since HDAds encode the adenoviral packaging signal, we hypothesized that the coadministration of Onc.Ad with HDAd would allow to be amplified and packaged during replication of Onc.Ad in transduced cancer cells. This combination could provide immunostimulation without losing oncolytic activity. We now show that coinfection of Onc.Ad with HDAd subsequently replicates HDAd vector DNA in trans in human cancer cell lines in vitro and in vivo, amplifying the transgenes the HDAd encode. This combinatorial treatment significantly suppresses the tumor growth compared to treatment with a single agent in an immunocompetent mouse model. Hence, combinatorial treatment of Onc.Ad with HDAd should overcome the inherent limitations of each agent and provide a highly immunogenic oncolytic therapy.

    General information

    StatePublished
    Ministry of Education publication typeA1 Journal article-refereed
    OrganisationsFaculty of Pharmacy , Division of Pharmaceutical Biosciences, Haartman Institute
    AuthorsFarzad, L., Cerullo, V., Yagyu, S., Bertin, T., Hemminki, A., Rooney, C., Lee, B., Suzuki, M.
    Number of pages1
    Pages14008
    Publication date2014
    Peer-reviewedYes

    Publication information

    JournalMolecular Therapy - Oncolytics
    Volume1
    ISSN (Print)2372-7705
    Original languageEnglish
    Fields of Science3111 Biomedicine
    Electronic versions
    DOIs
    SourceBibtex
    Source-IDurn:a2e21c865099f47e448336a592e34820

    Research output: Scientific - peer-reviewArticle

  3. Etunimikirja: suomalaiset nimitrendit 2000-luvulla

    General information

    StatePublished
    Ministry of Education publication typeE2 Popularised monograph
    OrganisationsDepartment of Finnish, Finno-Ugrian and Scandinavian Studies
    AuthorsSaarelma-Paukkala, M. M.
    Number of pages268
    Publication date2017

    Publication information

    Place of publicationHelsinki
    PublisherSuomalaisen Kirjallisuuden Seura
    ISBN (Print)978-952-222-831-4
    Original languageFinnish

    Publication series

    NameKirjokansi
    PublisherSuomalaisen Kirjallisuuden Seura
    Volume135
    Fields of Science6121 Languages, 5200 Other social sciences

    Research output: General publicBook

  4. Enterovirus-associated changes in blood transcriptomic profiles of children with genetic susceptibility to type 1 diabetes

    Aims/hypothesis Enterovirus infections have been associated with the development of type 1 diabetes in multiple studies, but little is known about enterovirus-induced responses in children at risk for developing type 1 diabetes. Our aim was to use genome-wide transcriptomics data to characterise enterovirus-associated changes in whole-blood samples from children with genetic susceptibility to type 1 diabetes.

    Methods Longitudinal whole-blood samples (356 samples in total) collected from 28 pairs of children at increased risk for developing type 1 diabetes were screened for the presence of enterovirus RNA. Seven of these samples were detected as enterovirus-positive, each of them collected from a different child, and transcriptomics data from these children were analysed to understand the individual-level responses associated with enterovirus infections. Transcript clusters with peaking or dropping expression at the time of enterovirus positivity were selected as the enterovirus-associated signals.

    Results Strong signs of activation of an interferon response were detected in four children at enterovirus positivity, while transcriptomic changes in the other three children indicated activation of adaptive immune responses. Additionally, a large proportion of the enterovirus-associated changes were specific to individuals. An enterovirus-induced signature was built using 339 genes peaking at enterovirus positivity in four of the children, and 77 of these genes were also upregulated in human peripheral blood mononuclear cells infected in vitro with different enteroviruses. These genes separated the four enterovirus-positive samples clearly from the remaining 352 blood samples analysed.

    Conclusions/interpretation We have, for the first time, identified enterovirus-associated transcriptomic profiles in whole-blood samples from children with genetic susceptibility to type 1 diabetes. Our results provide a starting point for understanding the individual responses to enterovirus infections in blood and their potential connection to the development of type 1 diabetes.

    General information

    StatePublished
    Ministry of Education publication typeA1 Journal article-refereed
    OrganisationsResearch Programs Unit, Diabetes and Obesity Research Program, Clinicum, Lastentautien yksikkö, Hospital for Children and Adolescents, University of Helsinki, University of Turku, University of Oulu, Turku University Hospital, Åbo Akademi University, University of Tampere, Pirkanmaa Hospital District, Folkhälsan Research Center, Tampere University Hospital (TAYS), Oulu University Hospital, University of Tampere
    AuthorsLietzen, N., An, L. T. T., Jaakkola, M. K., Kallionpää, H., Oikarinen, S., Mykkänen, J., Knip, M., Veijola, R., Ilonen, J., Toppari, J., Hyoty, H., Lahesmaa, R., Elo, L. L.
    Number of pages8
    Pages381-388
    Publication dateFeb 2018
    Peer-reviewedYes

    Publication information

    JournalDiabetologia
    Volume61
    Issue number2
    ISSN (Print)0012-186X
    Original languageEnglish
    Fields of ScienceClinical immunology, Enterovirus, Human, Microarray, Prediction and prevention of type 1 diabetes, HUMAN PANCREATIC-ISLETS, CAPSID PROTEIN VP1, COXSACKIEVIRUS B1, AUTOIMMUNITY, EXPRESSION, INFECTION, CELLS, RISK, POPULATION, INDUCTION, 3123 Gynaecology and paediatrics, 3121 Internal medicine, 3111 Biomedicine
    Electronic versions
    DOIs
    URLs
    SourceWOS
    Source-ID000419011600013

    Research output: Scientific - peer-reviewArticle

  5. The use of multiplex platforms for absolute and relative protein quantification of clinical material

    Abstract When introducing multiplex platforms to measure protein content in precious clinical material there is an increased risk of cross reactivity, loss of sensitivity as well as accuracy. In this paper, four multiplex platforms and one singleplex platform were compared by running pre- and post-treatment plasma samples from CML patients. We found a variation of absolute protein concentrations between platforms. For some of the analytes and platforms, relative differences between pre- and post-treatment samples correlated. We conclude that absolute concentrations measured by different platforms should be compared with caution and comparing relative differences could be more accurate.

    General information

    StatePublished
    Ministry of Education publication typeA1 Journal article-refereed
    OrganisationsDepartment of Medicine
    AuthorsChristiansson, L., Mustjoki, S., Simonsson, B., Olsson-Strömberg, U., Loskog, A. S., Mangsbo, S. M.
    Pages37 - 47
    Publication date2014
    Peer-reviewedYes

    Publication information

    JournalEuPA Open Proteomics
    Volume3
    ISSN (Print)2212-9685
    Original languageEnglish
    Fields of ScienceMultiplex protein quantification, ELISA, Luminex, Somalogic, Meso Scale Discovery, Chronic myeloid leukemia, 3111 Biomedicine
    Electronic versions
    DOIs
    SourceBibtex
    Source-IDurn:4e191989a667e19f8713a2476b2a37de

    Research output: Scientific - peer-reviewArticle

  6. Introduction: Nine Tales of the Field

    General information

    StatePublished
    Ministry of Education publication typeA3 Book chapter
    OrganisationsHumanistisen tiedekunnan kanslia
    AuthorsHirvi, L., Snellman, H.
    Number of pages14
    Pages7-20
    Publication date2012

    Host publication information

    Title of host publicationWhere is the Field?
    PublisherFinnish Literary Society
    ISBN (Print)978-952-222-388-3
    Fields of Science6160 Other humanities
    Electronic versions

    Research output: Scientific - peer-reviewChapter

  7. Viisaus, elämänkulku ja merkityksellisyys

    General information

    StatePublished
    OrganisationsFaculty of Theology
    AuthorsSpännäri, J.
    Publication date8 May 2017
    Peer-reviewedUnknown

    Publication information

    JournalTeologia.fi
    Original languageFinnish

    Research output: General publicOther articles

  8. Viisaus keskuudessamme – Elämänkokemus, kokemusviisaus ja merkityksellisyyden etsintä

    General information

    StatePublished
    OrganisationsFaculty of Theology
    AuthorsSpännäri, J.
    Publication date8 May 2017
    Peer-reviewedUnknown

    Publication information

    JournalTeologia.fi
    Original languageFinnish

    Research output: General publicOther articles

  9. Seize the day and seize seizures after cardiac arrest

    General information

    StatePublished
    Ministry of Education publication typeB1 Journal article
    OrganisationsClinicum, Diagnostic-Therapeutic Department, Anestesiologian yksikkö, University of Helsinki
    AuthorsSkrifvars, M. B., Hästbacka, J.
    Number of pages2
    PagesA3-A4
    Publication dateFeb 2018
    Peer-reviewedNo

    Publication information

    JournalResuscitation
    Volume123
    ISSN (Print)0300-9572
    Original languageEnglish
    Fields of ScienceBRAIN-INJURY, THERAPEUTIC HYPOTHERMIA, PROGNOSTIC-SIGNIFICANCE, TEMPERATURE MANAGEMENT, HEMODYNAMIC TARGETS, RESUSCITATION, METABOLISM, TRIAL, CARE, 3121 Internal medicine, 3126 Surgery, anesthesiology, intensive care, radiology
    DOIs
    SourceWOS
    Source-ID000419222600002

    Research output: ScientificEditorial

  10. ‘Aeromonas intestinalis’ and ‘Aeromonas enterica’ isolated from human faeces, ‘Aeromonas crassostreae’ from oyster and ‘Aeromonas aquatilis’ isolated from lake water represent novel species

    Abstract Four Aeromonas strains from clinical and environmental samples differed from known species on the basis of rpoD gene sequence. Multilocus phylogenetic analysis and in silico DNA-DNA hybridization confirmed them as four new species even though their 16S rRNA gene sequence similarity with their closest relatives was >98.7%, as occurred for other Aeromonas spp.

    General information

    StatePublished
    Ministry of Education publication typeA1 Journal article-refereed
    OrganisationsDepartment of Food and Environmental Sciences, Haartman Institute
    AuthorsFigueras, M., Latif-Eugenín, F., Ballester, F., Pujol, I., Tena, D., Berg, K., Hossain, M., Beaz-Hidalgo, R., Liles, M.
    Pages74 - 76
    Publication date2017
    Peer-reviewedYes

    Publication information

    JournalNew Microbes and New Infections
    Volume15
    ISSN (Print)2052-2975
    Original languageEnglish
    Fields of Science, faeces, new species, oysters, taxonomy, water, 1183 Plant biology, microbiology, virology
    Electronic versions
    DOIs
    SourceBibtex
    Source-IDurn:177055c2ea5ac50cbb3f79441e549d71

    Research output: Scientific - peer-reviewArticle

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